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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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CD83 Polyclonal Antibody
- 货号:YT0779
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human CD83. AA range:101-150
- 特异性:
- CD83 Polyclonal Antibody detects endogenous levels of CD83 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- CD83;CD83 antigen;hCD83;B-cell activation protein;Cell surface protein HB15;CD antigen CD83
- 背景:
- The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],
- 功能:
- function:May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.,online information:CD83 antigen,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subunit:Monomer.,tissue specificity:Expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells.,
- 细胞定位:
- Membrane; Single-pass type I membrane protein.
- 组织表达:
- Expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells.
- Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:400(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
- Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:400(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
- Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using CD83 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HepG2 cells, HUVEC cells, HeLa cells, and Jurkat cells, using CD83 Antibody. The lane on the right is blocked with the synthesized peptide.