CD130 Polyclonal Antibody
- 货号:YT0721
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 简介:
- >>Cytokine-cytokine receptor interaction;>>Viral protein interaction with cytokine and cytokine receptor;>>Signaling pathways regulating pluripotency of stem cells;>>JAK-STAT signaling pathway;>>Th17 cell differentiation;>>Kaposi sarcoma-associated herpesvirus infection;>>Coronavirus disease - COVID-19;>>Pathways in cancer;>>Viral carcinogenesis
- 蛋白名称:
- Interleukin-6 receptor subunit beta
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human CD130/gp130. AA range:748-797
- 特异性:
- CD130 Polyclonal Antibody detects endogenous levels of CD130 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- IL6ST;Interleukin-6 receptor subunit beta;IL-6 receptor subunit beta;IL-6R subunit beta;IL-6R-beta;IL-6RB;CDw130;Interleukin-6 signal transducer;Membrane glycoprotein 130;gp130;Oncostatin-M receptor subunit alpha;CD antigen CD130
- 背景:
- The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014],
- 功能:
- disease:Isoform 2 is an autoantigen found in rheumatoid arthritis (RA) but it is not specific to patients with RA.,domain:The box 1 motif is required for JAK interaction and/or activation.,domain:The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.,function:Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.,induction:Leukemia inhibitory factor (LIF) and Oncostatin-M (OSM) activate the type I OSM receptor while only
- 细胞定位:
- [Isoform 1]: Cell membrane ; Single-pass type I membrane protein .; [Isoform 2]: Secreted .
- 组织表达:
- Found in all the tissues and cell lines examined (PubMed:2261637). Expression not restricted to IL6 responsive cells (PubMed:2261637). ; [Isoform 2]: Expressed in blood serum (at protein level) (PubMed:24629561).
![](/uploadfiles/PIC/yt0721-wb.jpg)
- Western Blot analysis of 293T COLO cells using CD130 Polyclonal Antibody diluted at 1:500
![](/uploadfiles/PIC/yt0721_pic315718522.jpg)
- Immunofluorescence analysis of NIH/3T3 cells, using CD130/gp130 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt0721_pic315818522.jpg)
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CD130/gp130 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt0721_pic315918522.jpg)
- Western blot analysis of lysates from Jurkat cells, using CD130/gp130 Antibody. The lane on the right is blocked with the synthesized peptide.