CBP20 Polyclonal Antibody
- 货号：YT0696
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Nucleocytoplasmic transport;>>mRNA surveillance pathway;>>Spliceosome
- 蛋白名称：
- Nuclear cap-binding protein subunit 2
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human NCBP2. AA range:10-59
- 特异性：
- CBP20 Polyclonal Antibody detects endogenous levels of CBP20 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- NCBP2;CBP20;PIG55;Nuclear cap-binding protein subunit 2;20 kDa nuclear cap-binding protein;Cell proliferation-inducing gene 55 protein;NCBP 20 kDa subunit;CBP20;NCBP-interacting protein 1;NIP1
- 背景：
- The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
- 功能：
- function:Involved in mediating U snRNA export from the nucleus. Binds to 5' capped mRNA.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the RRM NCBP2 family.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Found in a U snRNA export complex with RNUXA/PHAX, NCBP1, NCBP2, RAN, XPO1 and m7G-capped RNA (By similarity). Heterodimer with NCBP1. Is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, UPF3B, UPF2, THOC4 and/or REFBP2.,
- 细胞定位：
- Nucleus . Cytoplasm .
- 组织表达：
- Cervix,Cervix carcinoma,Human small intestine,
- Western Blot analysis of various cells using CBP20 Polyclonal Antibody
- Western blot analysis of lysates from COLO205 cells, using NCBP2 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
