• 靶点：
• CB1

• 简介：
• >>Rap1 signaling pathway;>>Neuroactive ligand-receptor interaction;>>Thermogenesis;>>Retrograde endocannabinoid signaling

• 基因名称：
• CNR1

• 蛋白名称：
• Cannabinoid receptor 1

• Human Gene Id：
• 1268

• Human Swiss Prot No：
• P21554

• Mouse Gene Id：
• 12801

• Mouse Swiss Prot No：
• P47746

• Rat Gene Id：
• 25248

• Rat Swiss Prot No：
• P20272

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human CNR1. AA range:151-200

• 特异性：
• CB1 Polyclonal Antibody detects endogenous levels of CB1 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• CNR1;CNR;Cannabinoid receptor 1;CB-R;CB1;CANN6

• 实测条带：
• 53kD

• 背景：
• This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, May 2009],

• 功能：
• function:Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.,similarity:Belongs to the G-protein coupled receptor 1 family.,subunit:Interacts (via C-terminus) with CNRIP1.,tissue specificity:Widely expressed.,

• 细胞定位：
• Cell membrane ; Multi-pass membrane protein . Membrane raft . Mitochondrion outer membrane . Cell projection, axon . Cell junction, synapse, presynapse . Unexpectedly, in the mitochondria, the C-terminus is located in the mitochondrial intermembrane space, a compartment topologically considered as extracellular. In canonical seven-transmembrane G-protein coupled receptors, the C-terminus is cytosolic (By similarity). Found on presynaptic axon terminals in some GABAergic neurons in the somatosensory cortex (By similarity). .

• 组织表达：
• Widely expressed, with highest levels in fetal and adult brain. Expression levels of isoform 2 and isoform 3 are much lower than those of isoform 1.

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