Casein Kinase Iε Polyclonal Antibody
- 货号：YT0651
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>FoxO signaling pathway;>>Wnt signaling pathway;>>Hedgehog signaling pathway;>>Hippo signaling pathway;>>Hippo signaling pathway - multiple species;>>Circadian rhythm;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases
- 蛋白名称：
- Casein kinase I isoform epsilon
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human CKI-epsilon. AA range:276-325
- 特异性：
- Casein Kinase Iε Polyclonal Antibody detects endogenous levels of Casein Kinase Iε protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- CSNK1E;Casein kinase I isoform epsilon;CKI-epsilon;CKIe
- 背景：
- casein kinase 1 epsilon(CSNK1E) Homo sapiens The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014],
- 功能：
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation.,induction:Down-regulated during granulocytic differentiation.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer. Component of the circadian core oscillator, which includes the CRY pro
- 细胞定位：
- Cytoplasm . Nucleus .
- 组织表达：
- Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
- Western Blot analysis of LOVO cells using Casein Kinase Iε Polyclonal Antibody
- Immunofluorescence analysis of HeLa cells, using CKI-epsilon Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using CKI-epsilon Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from LOVO cells, using CKI-epsilon Antibody. The lane on the right is blocked with the synthesized peptide.
