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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
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CAS Polyclonal Antibody
- 货号:YT0641
- 应用:WB;IHC;IP;IF;ELISA
- 种属:Human;Rat;Mouse;
- 简介:
- >>Nucleocytoplasmic transport;>>Salmonella infection
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human CSE1L. AA range:1-50
- 特异性:
- CAS Polyclonal Antibody detects endogenous levels of CAS protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 ug:mg lysate. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- CSE1L;CAS;XPO2;Exportin-2;Exp2;Cellular apoptosis susceptibility protein;Chromosome segregation 1-like protein;Importin-alpha re-exporter
- 背景:
- Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012],
- 功能:
- function:Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.,sim
- 细胞定位:
- Cytoplasm . Nucleus . Shuttles between the nucleus and the cytoplasm. .
- 组织表达:
- Detected in brain, placenta, ovary, testis and trachea (at protein level) (PubMed:10331944). Widely expressed (PubMed:10331944). Highly expressed in testis and in proliferating cells (PubMed:7479798,PubMed:10331944).
![](/uploadfiles/PIC/yt0641-wb.jpg)
- Western Blot analysis of various cells using CAS Polyclonal Antibody diluted at 1:2000
![](/uploadfiles/PIC/yt0641_pic431218522.jpg)
- Immunofluorescence analysis of HeLa cells, using CSE1L Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt0641_pic431318522.jpg)
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CSE1L Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt0641_pic431418522.jpg)
- Western blot analysis of lysates from 293 cells, using CSE1L Antibody. The lane on the right is blocked with the synthesized peptide.