C/EBP ε Polyclonal Antibody
- 货号：YT0558
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 简介：
- >>Transcriptional misregulation in cancer;>>Acute myeloid leukemia
- 蛋白名称：
- CCAAT/enhancer-binding protein epsilon
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human C/EBP-epsilon. AA range:40-89
- 特异性：
- C/EBP ε Polyclonal Antibody detects endogenous levels of C/EBP ε protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- CEBPE;CCAAT/enhancer-binding protein epsilon;C/EBP epsilon
- 背景：
- The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008],
- 功能：
- function:C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.,online information:CEBPE mutation db,PTM:Phosphorylated.,similarity:Belongs to the bZIP family. C/EBP subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a dimer and can form stable heterodimers with C/EBP delta.,tissue specificity:Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines.,
- 组织表达：
- Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines.
- Western Blot analysis of various cells using C/EBP ε Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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- Western Blot analysis of Jurkat cells using C/EBP ε Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunofluorescence analysis of HeLa cells, using C/EBP-epsilon Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using C/EBP-epsilon Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from JurKat cells, treated with Insulin 0.01U/ml 15', using C/EBP-epsilon Antibody. The lane on the right is blocked with the synthesized peptide.
