BACH1 Polyclonal Antibody
- 货号：YT0431
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- Transcription regulator protein BACH1
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human BACH1. AA range:131-180
- 特异性：
- BACH1 Polyclonal Antibody detects endogenous levels of BACH1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- BACH1;Transcription regulator protein BACH1;BTB and CNC homolog 1;HA2303
- 背景：
- This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, May 2009],
- 功能：
- function:Transcriptional regulator that acts as repressor or activator. Binds, in-vitro, to NF-E2 binding sites. Play important roles in coordinating transcription activation and repression by MAFK.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. CNC subfamily.,similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 1 bZIP domain.,
- 组织表达：
- Epithelium,Placenta,Trachea,
- Western Blot analysis of various cells using BACH1 Polyclonal Antibody
- Immunofluorescence analysis of HeLa cells, using BACH1 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HepG2 cells, using BACH1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
