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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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AW-1 Polyclonal Antibody
- 货号:YT0425
- 应用:WB;IHC;IF;ELISA
- 种属:Human
- 蛋白名称:
- Zinc finger protein 174
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human ZNF174. AA range:201-250
- 特异性:
- AW-1 Polyclonal Antibody detects endogenous levels of AW-1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- ZNF174;ZSCAN8;Zinc finger protein 174;AW-1;Zinc finger and SCAN domain-containing protein 8
- 背景:
- function:Transcriptional repressor.,similarity:Belongs to the krueppel C2H2-type zinc-finger protein family.,similarity:Contains 1 SCAN box domain.,similarity:Contains 3 C2H2-type zinc fingers.,subunit:Homodimer.,tissue specificity:Expressed in a variety of organs, but most strongly in adult testis and ovary followed by small intestine, colon, prostate, thymus, spleen, pancreas, skeletal muscle, heart, brain and kidney. Also expressed in umbilical vein endothelial cells, foreskin fibroblast and HEPG2 cells.,
- 功能:
- function:Transcriptional repressor.,similarity:Belongs to the krueppel C2H2-type zinc-finger protein family.,similarity:Contains 1 SCAN box domain.,similarity:Contains 3 C2H2-type zinc fingers.,subunit:Homodimer.,tissue specificity:Expressed in a variety of organs, but most strongly in adult testis and ovary followed by small intestine, colon, prostate, thymus, spleen, pancreas, skeletal muscle, heart, brain and kidney. Also expressed in umbilical vein endothelial cells, foreskin fibroblast and HEPG2 cells.,
- 组织表达:
- Expressed in a variety of organs, but most strongly in adult testis and ovary followed by small intestine, colon, prostate, thymus, spleen, pancreas, skeletal muscle, heart, brain and kidney. Also expressed in umbilical vein endothelial cells, foreskin fibroblast and Hep-G2 cells.
- Western Blot analysis of various cells using AW-1 Polyclonal Antibody
- Immunofluorescence analysis of HUVEC cells, using ZNF174 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HeLa cells, using ZNF174 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).