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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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Aven Polyclonal Antibody
- 货号:YT0422
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Monkey
- 蛋白名称:
- Cell death regulator Aven
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human AVEN. AA range:301-350
- 特异性:
- Aven Polyclonal Antibody detects endogenous levels of Aven protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- AVEN;Cell death regulator Aven
- 背景:
- function:Protects against apoptosis mediated by Apaf-1.,subcellular location:Associated with intracellular membranes.,subunit:Binds Apaf-1, BCL-2 and BAD (Bcl-xl).,tissue specificity:Highly expressed in testis, ovary, thymus, prostate, spleen, small intestine, colon, heart, skeletal muscle, liver, kidney and pancreas.,
- 功能:
- function:Protects against apoptosis mediated by Apaf-1.,subcellular location:Associated with intracellular membranes.,subunit:Binds Apaf-1, BCL-2 and BAD (Bcl-xl).,tissue specificity:Highly expressed in testis, ovary, thymus, prostate, spleen, small intestine, colon, heart, skeletal muscle, liver, kidney and pancreas.,
- 细胞定位:
- Endomembrane system; Peripheral membrane protein. Associated with intracellular membranes.
- 组织表达:
- Highly expressed in testis, ovary, thymus, prostate, spleen, small intestine, colon, heart, skeletal muscle, liver, kidney and pancreas.
![](/uploadfiles/PIC/yt0422-wb.jpg)
- Western Blot analysis of various cells using Aven Polyclonal Antibody
![](/uploadfiles/PIC/yt0422_pic600318522.jpg)
- Western blot analysis of lysates from COS7 and LOVO cells, using AVEN Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/147xiaochangaicc.jpg)
- Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).