ATP5L2 Polyclonal Antibody
- 货号:YT0409
- 应用:WB;IF;ELISA
- 种属:Human
- 蛋白名称:
- ATP synthase subunit g 2 mitochondrial
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human ATP5L2. AA range:51-100
- 特异性:
- ATP5L2 Polyclonal Antibody detects endogenous levels of ATP5L2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- ATP5L2;ATP5K2;ATP synthase subunit g 2; mitochondrial;ATPase subunit g 2
- 背景:
- function:Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.,similarity:Belongs to the ATPase g subunit family.,subunit:F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(0) seems to have nine subunits: a, b, c, d, e, f, g, F6 and 8 (or A6L).,
- 功能:
- function:Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.,similarity:Belongs to the ATPase g subunit family.,subunit:F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(0) seems to have nine subunits: a, b, c,
- 细胞定位:
- Mitochondrion membrane .
![](/uploadfiles/PIC/yt0409_pic660718522.jpg)
- Immunofluorescence analysis of A549 cells, using ATP5L2 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt0409_pic660818522.jpg)
- Western blot analysis of lysates from A549 cells, using ATP5L2 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt0409_pic963218522.jpg)
- Western blot analysis of the lysates from HeLa cells using ATP5L2 antibody.