ATF-5 Polyclonal Antibody
- 货号：YT0388
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 蛋白名称：
- Cyclic AMP-dependent transcription factor ATF-5
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human ATF5. AA range:221-270
- 特异性：
- ATF-5 Polyclonal Antibody detects endogenous levels of ATF-5 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ATF5;ATFX;Cyclic AMP-dependent transcription factor ATF-5;cAMP-dependent transcription factor ATF-5;Activating transcription factor 5;Transcription factor ATFx
- 背景：
- function:Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4.,similarity:Belongs to the bZIP family.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a dimer. Interacts with PTP4A1/PRL-1 (By similarity). Interacts with CCND3, but not with CCND1 or CCND2.,
- 功能：
- function:Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4.,similarity:Belongs to the bZIP family.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a dimer. Interacts with PTP4A1/PRL-1 (By similarity). Interacts with CCND3, but not with CCND1 or CCND2.,
- 细胞定位：
- Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Actively transported to the centrosome and accumulated in the pericentriolar material (PCM) during G1 to M phase via a microtubule-dependent mechanism. During late telophase and cytokinesis, translocates from the centrosome to the midbody. .
- 组织表达：
- Widely expressed with higher expression levels in liver.
- Western Blot analysis of various cells using ATF-5 Polyclonal Antibody diluted at 1:1000
- Western blot analysis of lysates from Jurkat cells, using ATF5 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
