ApoL1 Polyclonal Antibody
- 货号：YT0277
- 应用：WB;IHC;IF;ELISA
- 种属：Human
- 简介：
- >>African trypanosomiasis
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human APOL1. AA range:261-310
- 特异性：
- ApoL1 Polyclonal Antibody detects endogenous levels of ApoL1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- APOL1;APOL;Apolipoprotein L1;Apolipoprotein L;Apo-L;ApoL;Apolipoprotein L-I;ApoL-I
- 背景：
- This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008],
- 功能：
- function:May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.,sequence caution:Translated as Gln.,similarity:Belongs to the apolipoprotein L family.,subunit:In plasma, interacts with APOA1 and mainly associated with large high density lipoprotein particles.,tissue specificity:Plasma. Found on APOA-I-containing high density lipoprotein (HDL3). Expressed in pancreas, lung, prostate, liver, placenta and spleen.,
- 组织表达：
- Plasma. Found on APOA-I-containing high density lipoprotein (HDL3). Expressed in pancreas, lung, prostate, liver, placenta and spleen.
- Western Blot analysis of various cells using ApoL1 Polyclonal Antibody diluted at 1:1000
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from A549 cells, using APOL1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from K562 cells using APOL1 antibody.
