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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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ANKT Polyclonal Antibody
- 货号:YT0233
- 应用:WB;ELISA
- 种属:Human;Rat;Mouse;
- 蛋白名称:
- Nucleolar and spindle-associated protein 1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human NUSAP1. AA range:392-441
- 特异性:
- ANKT Polyclonal Antibody detects endogenous levels of ANKT protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- NUSAP1;ANKT;BM-037;Nucleolar and spindle-associated protein 1;NuSAP
- 背景:
- NUSAP1 is a nucleolar-spindle-associated protein that plays a role in spindle microtubule organization (Raemaekers et al., 2003 [PubMed 12963707]).[supplied by OMIM, Jun 2009],
- 功能:
- domain:The KEN box is required for the FZR1-dependent degradation of this protein subsequent to ubiquitination.,function:Microtubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Ubiquitinated. Ubiquitination by FZR1 may lead to proteasome-dependent degradation of this protein.,similarity:Belongs to the NUSAP family.,subcellular location:Found in the cytoplasm and nucleolus during interphase and redistributes to the mitotic spindle in prometaphase (By similarity). Localizes to the mitotic spindle during anaphase and telophase then disappears from around the chromosomes during cytokinesis (By similarity). Localizes to multiple distinct regions of chromosomes throughout mitosis.,
- 细胞定位:
- Cytoplasm . Nucleus, nucleolus . Cytoplasm, cytoskeleton, spindle . Chromosome . Found in the cytoplasm and nucleolus during interphase and redistributes to the mitotic spindle in prometaphase (By similarity). Localizes to the mitotic spindle during anaphase and telophase then disappears from around the chromosomes during cytokinesis (By similarity). Localizes to multiple distinct regions of chromosomes throughout mitosis. .
- 组织表达:
- Bone marrow,Cervix,Epithelium,Fetal liver,Liver,Lung,Lymph,
![](/uploadfiles/PIC/yt0233-wb.jpg)
- Western Blot analysis of various cells using ANKT Polyclonal Antibody
![](/uploadfiles/PIC/yt0233_pic724118522.jpg)
- Western blot analysis of lysates from HT-29 cells, using NUSAP1 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt0233_pic1021218522.jpg)
- Western blot analysis of the lysates from HT-29 cells using NUSAP1 antibody.