Aldolase A Polyclonal Antibody
- 货号：YT0191
- 应用：WB;IF;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>Glycolysis / Gluconeogenesis;>>Pentose phosphate pathway;>>Fructose and mannose metabolism;>>Metabolic pathways;>>Carbon metabolism;>>Biosynthesis of amino acids;>>HIF-1 signaling pathway
- 蛋白名称：
- Fructose-bisphosphate aldolase A
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human ALDOA. AA range:1-50
- 特异性：
- Aldolase A Polyclonal Antibody detects endogenous levels of Aldolase A protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ALDOA;ALDA;Fructose-bisphosphate aldolase A;Lung cancer antigen NY-LU-1;Muscle-type aldolase
- 背景：
- The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Aug 2011],
- 功能：
- catalytic activity:D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.,disease:Defects in ALDOA are the cause of aldolase A deficiency [MIM:611881]; also known as aldoA deficiency or red cell aldolase deficiency. Aldolase A deficiency is an autosomal recessive disorder associated with hereditary hemolytic anemia.,miscellaneous:In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain.,pathway:Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.,similarity:Belongs to the class I fructose-bisphosphate aldolase family.,subunit:Homotetramer.,
- 细胞定位：
- Cytoplasm, myofibril, sarcomere, I band . Cytoplasm, myofibril, sarcomere, M line . In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca(2+). .
- 组织表达：
- Brain,Cajal-Retzius cell,Cervix,Colon carcinoma,Epithelium,Eye,Feta
- Western Blot analysis of various cells using Aldolase A Polyclonal Antibody diluted at 1:1000
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- Western Blot analysis of HT29 cells using Aldolase A Polyclonal Antibody diluted at 1:1000
- Western blot analysis of lysates from A549, HeLa, and HT-29 cells, using ALDOA Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HT-29 cells using ALDOA antibody.
