AKAP 250 Polyclonal Antibody
- 货号：YT0166
- 应用：IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- A-kinase anchor protein 12
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human AKAP12. AA range:301-350
- 特异性：
- AKAP 250 Polyclonal Antibody detects endogenous levels of AKAP 250 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- AKAP12;AKAP250;A-kinase anchor protein 12;AKAP-12;A-kinase anchor protein 250 kDa;AKAP 250;Gravin;Myasthenia gravis autoantigen
- 背景：
- The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
- 功能：
- caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,disease:Antibodies to the C-terminal of gravin can be produced by patients with myasthenia gravis (MG).,domain:Polybasic regions located between residues 266 and 557 are involved in binding PKC.,function:Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).,induction:Activated by lysophosphatidylcholine (lysoPC).,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 3 AKAP domains.,subcellular location:May be part of the cortical cytoskeleton.,subunit:Binds to dimeric RII-alpha regulatory subunit of PKC.,tissue specificity:Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood
- 细胞定位：
- Cytoplasm, cell cortex . Cytoplasm, cytoskeleton . Membrane ; Lipid-anchor . May be part of the cortical cytoskeleton.
- 组织表达：
- Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
- Immunofluorescence analysis of HeLa cells, using AKAP12 Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using AKAP12 Antibody. The picture on the right is blocked with the synthesized peptide.
