Adducin α/β Polyclonal Antibody

    • 货号:YT0125
    • 应用:WB;IHC;IP;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • Adducin α/β
      • 基因名称:
      • ADD1/ADD2
      • 蛋白名称:
      • Alpha-adducin/Beta-adducin
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human ADD1. AA range:688-737
      • 特异性:
      • Adducin α/β Polyclonal Antibody detects endogenous levels of Adducin α/β protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 ug:mg lysate. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • ADD1;ADDA;Alpha-adducin;Erythrocyte adducin subunit alpha;ADD2;ADDB;Beta-adducin;Erythrocyte adducin subunit beta
      • 实测条带:
      • 80kD
      • 背景:
      • adducin 1(ADD1) Homo sapiens Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008],
      • 功能:
      • alternative products:Additional isoforms seem to exist,domain:Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.,function:Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.,PTM:The N-terminus is blocked.,similarity:Belongs to the aldolase class II family. Adducin subfamily.,subunit:Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit. Binds ROCK1.,tissue specificity:Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.,
      • 细胞定位:
      • Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
      • 组织表达:
      • Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
      • 产品图片
      • Western Blot analysis of various cells using Adducin α/β Polyclonal Antibody diluted at 1:1000
      • Western Blot analysis of HELA cells using Adducin α/β Polyclonal Antibody diluted at 1:1000
      • Immunofluorescence analysis of HeLa cells, using ADD1 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ADD1 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HeLa cells, treated with Forskolin 40nM 30', using ADD1 Antibody. The lane on the right is blocked with the synthesized peptide.