ADCK4 Polyclonal Antibody
- 货号:YT0122
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Uncharacterized aarF domain-containing protein kinase 4
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human ADCK4. AA range:31-80
- 特异性:
- ADCK4 Polyclonal Antibody detects endogenous levels of ADCK4 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- ADCK4;Uncharacterized aarF domain-containing protein kinase 4
- 背景:
- This gene encodes a protein with two copies of a domain found in protein kinases. The encoded protein has a complete protein kinase catalytic domain, and a truncated domain that contains only the active and binding sites of the protein kinase domain, however, it is not known whether the protein has any kinase activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
- 功能:
- function:The function of this protein is not yet clear. It is not known if it has protein kinase activity and what type of substrate it would phosphorylate (Ser, Thr or Tyr).,similarity:Belongs to the protein kinase superfamily. ADCK protein kinase family.,similarity:Contains 1 protein kinase domain.,
- 细胞定位:
- Mitochondrion membrane ; Single-pass membrane protein . Cytoplasm, cytosol . Cell membrane .
- 组织表达:
- Widely expressed, including renal podocytes.
- Western Blot analysis of various cells using ADCK4 Polyclonal Antibody
- Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Immunofluorescence analysis of A549 cells, using ADCK4 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HepG2 cells, using ADCK4 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using ADCK4 antibody.