• 靶点：
• ADAR1

• 简介：
• >>Cytosolic DNA-sensing pathway;>>Measles;>>Influenza A;>>Coronavirus disease - COVID-19

• 基因名称：
• ADAR

• 蛋白名称：
• Double-stranded RNA-specific adenosine deaminase

• Human Gene Id：
• 103

• Human Swiss Prot No：
• P55265

• Mouse Gene Id：
• 56417

• Mouse Swiss Prot No：
• Q99MU3

• Rat Gene Id：
• 81635

• Rat Swiss Prot No：
• P55266

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human ADAR1. AA range:1172-1221

• 特异性：
• ADAR1 Polyclonal Antibody detects endogenous levels of ADAR1 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• ADAR;ADAR1;DSRAD;G1P1;IFI4;Double-stranded RNA-specific adenosine deaminase;DRADA;136 kDa double-stranded RNA-binding protein;p136;Interferon-inducible protein 4;IFI-4;K88DSRBP

• 实测条带：
• 135kD

• 背景：
• adenosine deaminase, RNA specific(ADAR) Homo sapiens This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010],

• 功能：
• caution:The N-terminus of isoform 4 has been derived from EST and genomic sequences.,disease:Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH) [MIM:127400]; also known as reticulate acropigmentation of Dohi. DSH is a pigmentary genodermatosis of autosomal dominant inheritance characterized by a mixture of hyperpigmented and hypopigmented macules distributed on the dorsal parts of the hands and feet.,function:Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutama

• 细胞定位：
• [Isoform 1]: Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus (PubMed:7565688, PubMed:24753571). Nuclear import is mediated by TNPO1 (PubMed:24753571). .; [Isoform 5]: Cytoplasm . Nucleus . Nucleus, nucleolus . Predominantly nuclear but can shuttle between nucleus and cytoplasm. TNPO1 can mediate its nuclear import whereas XPO5 can mediate its nuclear export. .

• 组织表达：
• Ubiquitously expressed, highest levels were found in brain and lung (PubMed:7972084). Isoform 5 is expressed at higher levels in astrocytomas as compared to normal brain tissue and expression increases strikingly with the severity of the tumor, being higher in the most aggressive tumors.

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