ACTR-IB Polyclonal Antibody
- 货号：YT0106
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>Cytokine-cytokine receptor interaction;>>TGF-beta signaling pathway;>>Signaling pathways regulating pluripotency of stem cells
- 蛋白名称：
- Activin receptor type-1B
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human ACV1B. AA range:73-122
- 特异性：
- ACTR-IB Polyclonal Antibody detects endogenous levels of ACTR-IB protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ACVR1B;ACVRLK4;ALK4;Activin receptor type-1B;Activin receptor type IB;ACTR-IB;Activin receptor-like kinase 4;ALK-4;Serine/threonine-protein kinase receptor R2;SKR2
- 背景：
- This gene encodes an activin A type IB receptor. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I and two type II receptors. This protein is a type I receptor which is essential for signaling. Mutations in this gene are associated with pituitary tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010],
- 功能：
- catalytic activity:ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.,cofactor:Magnesium or manganese.,function:On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Phosphorylates TTRAP.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.,similarity:Contains 1 GS domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with AIP1. Part of a complex consisting of AIP1, ACVR2A, ACVR1B and SMAD3. Interacts with TTRAP.,tissue specificity:Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver.,
- 细胞定位：
- Cell membrane ; Single-pass type I membrane protein .
- 组织表达：
- Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver.
- Western Blot analysis of various cells using ACTR-IB Polyclonal Antibody diluted at 1:1000
- Immunofluorescence analysis of HeLa cells, using ACV1B Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from 293 and mouse liver cells, using ACV1B Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
