ACOT2 Polyclonal Antibody
- 货号：YT0087
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 简介：
- >>Fatty acid elongation;>>Biosynthesis of unsaturated fatty acids;>>Metabolic pathways;>>Ovarian steroidogenesis
- 蛋白名称：
- Acyl-coenzyme A thioesterase 2 mitochondrial
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human ACOT2. AA range:171-220
- 特异性：
- ACOT2 Polyclonal Antibody detects endogenous levels of ACOT2 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ACOT2;PTE2;PTE2A;Acyl-coenzyme A thioesterase 2; mitochondrial;Acyl-CoA thioesterase 2;Acyl-coenzyme A thioester hydrolase 2a;CTE-Ia;Long-chain acyl-CoA thioesterase 2;ZAP128
- 背景：
- This gene encodes a member of the acyl-CoA thioesterase protein family, and is one of four acyl-CoA hydrolase genes located in a cluster on chromosome 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012],
- 功能：
- catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,caution:Was originally (PubMed:10944470) thought to be peroxisomal but was later shown (PubMed:16940157) to be mitochondrial.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Displays high levels of activity on medium- and long chain acyl CoAs.,similarity:Belongs to the C/M/P thioester hydrolase family.,tissue specificity:Strongest expression in heart, liver, muscle and kidney. Weak in placenta and pancreas.,
- 组织表达：
- Strongest expression in heart, liver, muscle and kidney. Weak in placenta and pancreas.
- Western Blot analysis of VEC cells using ACOT2 Polyclonal Antibody diluted at 1:500
- Western blot analysis of lysates from Jurkat and HUVEC cells, using ACOT2 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HepG2 cells using ACOT2 antibody.
