ACOT12 Polyclonal Antibody
- 货号:YT0086
- 应用:IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>Pyruvate metabolism;>>Metabolic pathways
- 蛋白名称:
- Acyl-coenzyme A thioesterase 12
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human ACOT12. AA range:281-330
- 特异性:
- ACOT12 Polyclonal Antibody detects endogenous levels of ACOT12 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- ACOT12;CACH;CACH1;STARD15;Acyl-coenzyme A thioesterase 12;Acyl-CoA thioesterase 12;Acyl-CoA thioester hydrolase 12;Cytoplasmic acetyl-CoA hydrolase 1;CACH-1;hCACH-1;START domain-containing protein 15;StARD15
- 背景:
- catalytic activity:Acetyl-CoA + H(2)O = CoA + acetate.,function:Hydrolyzes acetyl-CoA to acetate and CoA.,pathway:Carbohydrate metabolism; pyruvate metabolism.,similarity:Contains 1 START domain.,similarity:Contains 2 acyl coenzyme A hydrolase domains.,subunit:Homodimer or homotetramer.,
- 功能:
- catalytic activity:Acetyl-CoA + H(2)O = CoA + acetate.,function:Hydrolyzes acetyl-CoA to acetate and CoA.,pathway:Carbohydrate metabolism; pyruvate metabolism.,similarity:Contains 1 START domain.,similarity:Contains 2 acyl coenzyme A hydrolase domains.,subunit:Homodimer or homotetramer.,
- 细胞定位:
- Cytoplasm, cytosol .
- 组织表达:
- Chondrosarcoma Lung Metastasis,Liver,
- Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Immunofluorescence analysis of A549 cells, using ACOT12 Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using ACOT12 Antibody. The picture on the right is blocked with the synthesized peptide.