ACOT1 Polyclonal Antibody
- 货号:YT0085
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Rat;Mouse;
- 简介:
- >>Fatty acid elongation;>>Biosynthesis of unsaturated fatty acids;>>Metabolic pathways;>>Ovarian steroidogenesis
- 蛋白名称:
- Acyl-coenzyme A thioesterase 1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human ACOT1. AA range:91-140
- 特异性:
- ACOT1 Polyclonal Antibody detects endogenous levels of ACOT1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- ACOT1;CTE1;Acyl-coenzyme A thioesterase 1;Acyl-CoA thioesterase 1;CTE-I;CTE-Ib;Inducible cytosolic acyl-coenzyme A thioester hydrolase;Long chain acyl-CoA thioester hydrolase;Long chain acyl-CoA hydrolase
- 背景:
- catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16.,similarity:Belongs to the C/M/P thioester hydrolase family.,subunit:Monomer.,
- 功能:
- catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16.,similarity:Belongs to the C/M/P thioester hydrolase family.,subunit:Monomer.,
- 细胞定位:
- Cytoplasm, cytosol .
![](/uploadfiles/PIC/yt0085_pic650718522.jpg)
- Immunofluorescence analysis of MCF7 cells, using ACOT1 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt0085_pic650818522.jpg)
- Western blot analysis of lysates from Jurkat cells, using ACOT1 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt0085_pic955218522.jpg)
- Western blot analysis of the lysates from HT-29 cells using ACOT1 antibody.