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- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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- Western blot analysis of lysates from Hela cells.
Primary antibody: mouse anti GAPDH(cat:YM3029 1:5000),rabbit anti GAPDH(cat:YM8016 1:5000).
Secondary antibody:goat anti mouse(cat:RS0001 1:20000),goat anti rabbit(cat:RS0002 1:20000).
Exposure ECL: catalog: YS0124 super ECL and YS0123 enhance ECL, 10second