G3BP-1 (Phospho Ser149) Rabbit pAb
- 货号：YP1870
- 应用：IHC;WB
- 种属：Human;Mouse
- 蛋白名称：
- Ras GTPase-activating protein-binding protein 1 (G3BP-1) (EC 3.6.4.12) (EC 3.6.4.13) (ATP-dependent DNA helicase VIII) (hDH VIII) (GAP SH3 domain-binding protein 1)
- 免疫原：
- Synthesized peptide derived from human G3BP-1 (Phospho Ser149)
- 特异性：
- This antibody detects endogenous levels of G3BP-1 (Phospho Ser149) Rabbit pAb at Human, Mouse
- 组成：
- Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
- 稀释：
- WB 1:500-2000 IHC 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- Ras GTPase-activating protein-binding protein 1 (G3BP-1) (EC 3.6.4.12) (EC 3.6.4.13) (ATP-dependent DNA helicase VIII) (hDH VIII) (GAP SH3 domain-binding protein 1)
- 背景：
- G3BP stress granule assembly factor 1(G3BP1) Homo sapiens This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008],
- 功能：
- cofactor:Magnesium. Required for helicase activity.,domain:The NTF2 domain mediates multimerization.,function:May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.,PTM:Arg-435 is dimethylated, probably to asymmetric dimethylarginine.,PTM:Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a
- 细胞定位：
- Cytoplasm, cytosol . Perikaryon . Cytoplasm, Stress granule . Nucleus . Cytoplasmic in proliferating cells (PubMed:11604510). Cytosolic and partially nuclear in resting cells (PubMed:11604510). Recruited to stress granules in response to arsenite treatment (PubMed:12642610, PubMed:20180778). The unphosphorylated form is recruited to stress granules (PubMed:12642610). HRAS signaling contributes to this process by regulating G3BP dephosphorylation (PubMed:12642610). .
- Western Blot analysis of HeLa mouse brain tissue using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 25°C，1.5hours
