CSK (Phospho Tyr304) Rabbit pAb
- 货号：YP1858
- 应用：IHC;WB
- 种属：Human;Mouse;Rat
- 简介：
- >>Epithelial cell signaling in Helicobacter pylori infection
- 蛋白名称：
- Tyrosine-protein kinase CSK (EC 2.7.10.2) (C-Src kinase) (Protein-tyrosine kinase CYL)
- 免疫原：
- Synthesized peptide derived from human CSK (Phospho Tyr304)
- 特异性：
- This antibody detects endogenous levels of CSK (Phospho Tyr304) Rabbit pAb at Human, Mouse,Rat
- 组成：
- Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
- 稀释：
- WB 1:500-2000 IHC 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- Tyrosine-protein kinase CSK (EC 2.7.10.2) (C-Src kinase) (Protein-tyrosine kinase CYL)
- 功能：
- catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.,PTM:Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Mainly cytoplasmic, also present in lipid rafts.,subunit:Interacts with PTPN8 (By similarity). Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1.,tissue specificity:Expressed in lung and macrophages.,
- 细胞定位：
- Cytoplasm . Cell membrane . Mainly cytoplasmic, also present in lipid rafts. .
- 组织表达：
- Expressed in lung and macrophages.
- Western Blot analysis of 1 MCF-7 cell, 2 LPS 100ng/mL 30min treated ,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
