• 靶点：
• PDE4D

• 简介：
• >>Purine metabolism;>>Metabolic pathways;>>cAMP signaling pathway;>>Parathyroid hormone synthesis, secretion and action;>>Morphine addiction

• 基因名称：
• PDE4D DPDE3

• 蛋白名称：
• PDE4D (Phospho-Ser578)

• Human Gene Id：
• 5144

• Human Swiss Prot No：
• Q08499

• Mouse Gene Id：
• 238871

• Mouse Swiss Prot No：
• Q01063

• Rat Gene Id：
• 24627

• Rat Swiss Prot No：
• P14270

• 免疫原：
• Synthesized peptide derived from human PDE4D (Phospho-Ser578)

• 特异性：
• This antibody detects endogenous levels of PDE4D (Phospho-Ser578) at Human, Mouse,Rat

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500-2000

• 纯化工艺：
• The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• cAMP-specific 3',5'-cyclic phosphodiesterase 4D (EC 3.1.4.17) (DPDE3) (PDE43)

• 实测条带：
• 76kD

• 背景：
• This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009],

• 功能：
• catalytic activity:Adenosine 3',5'-cyclic phosphate + H(2)O = adenosine 5'-phosphate.,cofactor:Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.,disease:Genetic variations in PDE4D might be associated with susceptibility to stroke type 1 (STRK1) [MIM:606799]. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. PubMed:17006457 states that association with stroke has to be considered with caution.,enzyme regulation:Inhibited by rolipram. Activated by phosphatidic acid.,function:Regulates the levels of cAMP in the cell.,pathway:Purine metabolism; cAMP degradation; AMP from cAMP: step 1/1.,PTM:Isoform 2 and isoform 11 are activated by phosphorylation (in vitro), but not isoform 8. Isoform 7 a

• 细胞定位：
• Apical cell membrane . Cytoplasm . Membrane . Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome (By similarity). Colocalized with SHANK2 to the apical membrane of colonic crypt cells. .

• 组织表达：
• Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. ; [Isoform 6]: Detected in brain. ; [Isoform 8]: Detected in brain, placenta, lung and kidney. ; [Isoform 7]: Detected in heart and skeletal muscle.

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