XRCC1 (Phospho Thr284) rabbit pAb
- 货号：YP1744
- 应用：WB
- 种属：Human;Mouse;Rat
- 简介：
- >>Base excision repair
- 蛋白名称：
- XRCC1 (Phospho-Thr284)
- 免疫原：
- Synthesized peptide derived from human XRCC1 (Phospho-Thr284)
- 特异性：
- This antibody detects endogenous levels of XRCC1 (Phospho-Thr284) at Human, Mouse,Rat
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 纯化工艺：
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- DNA repair protein XRCC1 (X-ray repair cross-complementing protein 1)
- 背景：
- The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008],
- 功能：
- function:Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.,polymorphism:Carriers of the polymorphic Gln-399 allele may be at greater risk for tobacco- and age-related DNA damage.,PTM:Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.,PTM:Sumoylated.,similarity:Contains 2 BRCT domains.,subcellular location:Accumulates at sites of DNA damage.,subunit:Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF.,
- 细胞定位：
- Nucleus . Moves from the nucleoli to the global nuclear chromatin upon DNA damage. .
- 组织表达：
- Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level).
- Western Blot analysis of 1 HeLa cell, 2 LPS 100ng/mL 30min treated ,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
