ILK-1 (Phospho Ser343) rabbit pAb
- 货号：YP1702
- 应用：WB
- 种属：Human;Mouse;Rat
- 简介：
- >>PPAR signaling pathway;>>Axon guidance;>>Focal adhesion;>>Bacterial invasion of epithelial cells;>>Shigellosis;>>Endometrial cancer
- 蛋白名称：
- ILK-1 (Phospho-Ser343)
- 免疫原：
- Synthesized peptide derived from human ILK-1 (Phospho-Ser343)
- 特异性：
- This antibody detects endogenous levels of ILK-1 (Phospho-Ser343) at Human, Mouse,Rat
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 纯化工艺：
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- Integrin-linked protein kinase (EC 2.7.11.1) (59 kDa serine/threonine-protein kinase) (ILK-1) (ILK-2) (p59ILK)
- 背景：
- This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013],
- 功能：
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:A PH-like domain is involved in phosphatidylinositol phosphate binding.,enzyme regulation:Stimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K-dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK.,function:Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also
- 细胞定位：
- Cell junction, focal adhesion . Cell membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, lamellipodium . Cytoplasm, myofibril, sarcomere .
- 组织表达：
- Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
- Western Blot analysis of 1 HEK-293 cell, 2 Serum-free treated ,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
