• 靶点：
• SPHK1

• 简介：
• >>Sphingolipid metabolism;>>Metabolic pathways;>>Calcium signaling pathway;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Fc gamma R-mediated phagocytosis;>>Tuberculosis

• 基因名称：
• SPHK1 SPHK SPK

• 蛋白名称：
• SPHK1 (Phospho-Ser225)

• Human Gene Id：
• 8877

• Human Swiss Prot No：
• Q9NYA1

• Mouse Gene Id：
• 20698

• Mouse Swiss Prot No：
• Q8CI15

• Rat Gene Id：
• 170897

• Rat Swiss Prot No：
• Q91V26

• 免疫原：
• Synthesized peptide derived from human SPHK1 (Phospho-Ser225)

• 特异性：
• This antibody detects endogenous levels of SPHK1 (Phospho-Ser225) at Human, Mouse,Rat

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500-2000

• 纯化工艺：
• The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• Sphingosine kinase 1 (SK 1) (SPK 1) (EC 2.7.1.91)

• 实测条带：
• 42kD

• 背景：
• The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

• 功能：
• catalytic activity:ATP + sphinganine = ADP + sphinganine 1-phosphate.,catalytic activity:ATP + sphingosine = ADP + sphingosine 1-phosphate.,cofactor:Magnesium.,function:Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.,similarity:Contains 1 DAGKc domain.,subunit:Interacts with ACY1 (By similarity). Binds to calmodulin. Interacts with SPHKAP.,tissue specificity:Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.,

• 细胞定位：
• Cytoplasm . Nucleus . Cell membrane . Endosome membrane ; Peripheral membrane protein . Membrane, clathrin-coated pit . Cell junction, synapse . Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner (PubMed:19854831). Binds to membranes containing negatively charged lipids but not neutral lipids (PubMed:24929359). Recruited to endocytic membranes by sphingosine where promotes membrane fusion (By similarity). .

• 组织表达：
• Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Expressed in brain cortex (at protein level) (PubMed:29662056).

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