L-plastin (Phospho Ser5) rabbit pAb
- 货号：YP1681
- 应用：WB
- 种属：Human;Mouse;Rat
- 蛋白名称：
- L-plastin (Phospho-Ser5)
- 免疫原：
- Synthesized peptide derived from human L-plastin (Phospho-Ser5)
- 特异性：
- This antibody detects endogenous levels of L-plastin (Phospho-Ser5) at Human, Mouse,Rat
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 纯化工艺：
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- Plastin-2 (L-plastin) (LC64P) (Lymphocyte cytosolic protein 1) (LCP-1)
- 背景：
- Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. [provided by RefSeq, Jul 2008],
- 功能：
- function:Actin-binding protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia.,PTM:Phosphorylated.,PTM:The N-terminus is blocked.,similarity:Contains 2 actin-binding domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 CH (calponin-homology) domains.,subunit:Monomer.,tissue specificity:Restricted to the spleen and other lymph node-containing organs. Expressed in neutrophils, monocytes, B lymphocytes, and myeloid cells.,
- 细胞定位：
- Cytoplasm, cytoskeleton . Cell junction . Cell projection . Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Relocalizes to the immunological synapse between peripheral blood T-lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28 (PubMed:17294403). Associated with the actin cytoskeleton at membrane ruffles. Relocalizes to actin-rich cell projections upon serine phosphorylation (PubMed:16636079). .
- 组织表达：
- Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T-lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells.
- Western Blot analysis of 1 K562 cell, 2 LPS 100ng/mL 30min treated ,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
