CTIP (Phospho Ser327) rabbit pAb
- 货号：YP1560
- 应用：WB;ELISA
- 种属：Human;Mouse
- 简介：
- >>Homologous recombination
- 蛋白名称：
- CTIP (Phospho Ser327)
- 免疫原：
- Synthesized peptide derived from human CTIP (Phospho Ser327)
- 特异性：
- This antibody detects endogenous levels of Human,Mouse CTIP (Phospho Ser327)
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:1000-2000 ELISA 1:5000-20000
- 纯化工艺：
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- DNA endonuclease RBBP8 (EC 3.1.-.-;CtBP-interacting protein;CtIP;Retinoblastoma-binding protein 8;RBBP-8;Retinoblastoma-interacting protein and myosin-like;RIM;Sporulation in the absence of SPO11 protein 2 homolog;SAE2)
- 背景：
- The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008],
- 功能：
- function:May modulate the functions ascribed to BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1.,PTM:Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.,subcellular location:Predominantly nuclear.,subunit:Interacts with CTBP, with the C-terminal (BRCT) domains of BRCA1, and with the retinoblastoma protein.,
- 细胞定位：
- Nucleus . Chromosome . Associates with sites of DNA damage in S/G2 phase (PubMed:10764811, PubMed:25349192). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604). .
- 组织表达：
- Expressed in ER-positive breast cancer lines, but tends to be down-regulated ER-negative cells (at protein level).
- Western Blot analysis of 1 A431 treated with LPS, 2 A431,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
