MOK (Phospho Tyr161) Polyclonal Antibody
- 货号：YP1236
- 应用：IHC;IF;WB
- 种属：Human;Mouse;Rat
- 蛋白名称：
- MOK (Phospho-Tyr161)
- 免疫原：
- Synthesized peptide derived from human MOK (Phospho-Tyr161)
- 特异性：
- This antibody detects endogenous phospho levels of MOK (Phospho-Tyr161) at Human:Y161, Mouse:Y161, Rat:Y161
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- IHC 1:50-200, WB 1:500-2000. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- MAPK/MAK/MRK overlapping kinase (EC 2.7.11.22;MOK protein kinase;Renal tumor antigen 1;RAGE-1)
- 背景：
- MOK protein kinase(MOK) Homo sapiens This gene belongs to the MAP kinase superfamily. The gene was found to be regulated by caudal type transcription factor 2 (Cdx2) protein. The encoded protein, which is localized to epithelial cells in the intestinal crypt, may play a role in growth arrest and differentiation of cells of upper crypt and lower villus regions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Dec 2012],
- 功能：
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Phosphorylation appears to increase the enzymatic activity.,function:Able to phosphorylate several exogenous substrates and to undergo autophosphorylation.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Expressed in heart, brain, lung, kidney, and pancreas, and at very low levels in placenta, liver and skeletal muscle. Detected in retina.,
- 细胞定位：
- Cytoplasm . Cell projection, cilium . Nucleus .
- 组织表达：
- Expressed in heart, brain, lung, kidney, and pancreas, and at very low levels in placenta, liver and skeletal muscle. Detected in retina.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
- Western Blot analysis of 1 HeLa cell, 2 LPS 100ng/mL 30min treated ,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
