CAD (phospho Thr456) Polyclonal Antibody

    • 货号:YP1083
    • 应用:IHC;IF;ELISA
    • 种属:Human;Mouse
      • 靶点:
      • CAD
      • 简介:
      • >>Pyrimidine metabolism;>>Alanine, aspartate and glutamate metabolism;>>Metabolic pathways;>>Biosynthesis of cofactors
      • 基因名称:
      • CAD
      • 蛋白名称:
      • CAD protein
      • Human Gene Id:
      • 790
      • Human Swiss Prot No:
      • P27708
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human CAD around the phosphorylation site of Thr456. AA range:422-471
      • 特异性:
      • Phospho-CAD (T456) Polyclonal Antibody detects endogenous levels of CAD protein only when phosphorylated at T456.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • CAD;CAD protein
      • 分子量:
      • 243kD
      • 背景:
      • The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015],
      • 功能:
      • catalytic activity:(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.,catalytic activity:2 ATP + L-glutamine + HCO(3)(-) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.,catalytic activity:Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.,cofactor:Binds 1 zinc ion per subunit (for dihydroorotase activity) .,enzyme regulation:Allosterically regulated and controlled by phosphorylation. 5-phosphoribose 1-diphosphate is an activator while UMP is an inhibitor of the CPSase reaction.,function:This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).,miscellaneous:GATase (glutamine amidotransferase) and CPSase (carbamoyl phosphate synthase) form together the glutamine-dependent CPSase (GD-CPSase) (EC 6.3.5.5).,online information:Aspartate carbamoyltransferase entry,pathway:Pyrimi
      • 细胞定位:
      • Cytoplasm . Nucleus . Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth.
      • 组织表达:
      • Colon adenocarcinoma,Epithe
      • 产品图片
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CAD (Phospho-Thr456) Antibody
      • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using CAD (Phospho-Thr456) Antibody. The picture on the right is blocked with the phospho peptide.