• 靶点：
• AIP4

• 简介：
• >>Ubiquitin mediated proteolysis;>>Endocytosis;>>TNF signaling pathway;>>Non-alcoholic fatty liver disease

• 基因名称：
• ITCH

• 蛋白名称：
• E3 ubiquitin-protein ligase Itchy homolog

• Human Gene Id：
• 83737

• Human Swiss Prot No：
• Q96J02

• Mouse Gene Id：
• 16396

• Mouse Swiss Prot No：
• Q8C863

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human ITCH around the phosphorylation site of Tyr420. AA range:386-435

• 特异性：
• Phospho-AIP4 (Y420) Polyclonal Antibody detects endogenous levels of AIP4 protein only when phosphorylated at Y420.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• ITCH;E3 ubiquitin-protein ligase Itchy homolog;Itch;Atrophin-1-interacting protein 4;AIP4;NFE2-associated polypeptide 1;NAPP1

• 分子量：
• 103kD

• 背景：
• itchy E3 ubiquitin protein ligase(ITCH) Homo sapiens This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012],

• 功能：
• function:E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated on tyrosine residues.,similarity:Contains 1 C2 domain.,similarity:Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.,similarity:Contains 4 WW domains.,subunit:Interacts via its WW domains with DRPLA, NFE2 and CBLC. Interacts with Epstein-Barr virus LMP2A. Interacts with NOTCH1, OCLN, JUN and JUNB. Interacts with NDFIP1 in vitro (By similarity). Interacts with ARHGEF7.,tissue specificity:Widely expressed.,

• 细胞定位：
• Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm . Nucleus . Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . May be recruited to exosomes by NDFIP1 (PubMed:18819914). Localizes to plasma membrane upon CXCL12 stimulation where it co-localizes with CXCL4 (PubMed:14602072). Localization to early endosomes is increased upon CXCL12 stimulation where it co-localizes with DTX3L and CXCL4 (PubMed:24790097). .

• 组织表达：
• Widely expressed.

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