Myt 1 (phospho Ser83) Polyclonal Antibody
- 货号:YP1052
- 应用:IHC;IF;ELISA
- 种属:Human;Rat;Mouse;
- 靶点:
- Myt 1
- 简介:
- >>Cell cycle;>>Oocyte meiosis;>>Progesterone-mediated oocyte maturation
- 基因名称:
- PKMYT1
- 蛋白名称:
- Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
- Human Gene Id:
- 9088
- Human Swiss Prot No:
- Q99640
- Mouse Swiss Prot No:
- Q9ESG9
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human MYT1 around the phosphorylation site of Ser83. AA range:49-98
- 特异性:
- Phospho-Myt 1 (S83) Polyclonal Antibody detects endogenous levels of Myt 1 protein only when phosphorylated at S83.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- PKMYT1;MYT1;Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase;Myt1 kinase
- 分子量:
- 55kD
- 背景:
- This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.,enzyme regulation:Negatively regulated by hyperphosphorylation during mitosis. The hyperphosphorylated form does not associate with CCNB1-CDC2 complexes. The PLK1 protein kinase may be required for mitotic phosphorylation.,function:Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the cdc2 kinase specifically when cdc2 is complexed to cyclins. Mediates phosphorylation of cdc2 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of cdc2 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be ind
- 细胞定位:
- Endoplasmic reticulum membrane ; Peripheral membrane protein . Golgi apparatus membrane ; Peripheral membrane protein .
- 组织表达:
- Brain,Epithelium,PCR rescued clones,
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- 产品图片
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MYT1 (Phospho-Ser83) Antibody
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MYT1 (Phospho-Ser83) Antibody. The picture on the right is blocked with the phospho peptide.