• 靶点：
• ILK

• 简介：
• >>PPAR signaling pathway;>>Axon guidance;>>Focal adhesion;>>Bacterial invasion of epithelial cells;>>Shigellosis;>>Endometrial cancer

• 基因名称：
• ILK

• 蛋白名称：
• Integrin-linked protein kinase

• Human Gene Id：
• 3611

• Human Swiss Prot No：
• Q13418

• Mouse Gene Id：
• 16202

• Mouse Swiss Prot No：
• O55222

• Rat Gene Id：
• 170922

• Rat Swiss Prot No：
• Q99J82

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human ILK around the phosphorylation site of Ser246. AA range:212-261

• 特异性：
• Phospho-ILK (S246) Polyclonal Antibody detects endogenous levels of ILK protein only when phosphorylated at S246.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• ILK;ILK1;ILK2;Integrin-linked protein kinase;59 kDa serine/threonine-protein kinase;ILK-1;ILK-2;p59ILK

• 分子量：
• 51kD

• 背景：
• This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013],

• 功能：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:A PH-like domain is involved in phosphatidylinositol phosphate binding.,enzyme regulation:Stimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K-dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK.,function:Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also

• 细胞定位：
• Cell junction, focal adhesion . Cell membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, lamellipodium . Cytoplasm, myofibril, sarcomere .

• 组织表达：
• Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.

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