• 靶点：
• DNA Ligase IV

• 简介：
• >>Non-homologous end-joining

• 基因名称：
• LIG4

• 蛋白名称：
• DNA ligase 4

• Human Gene Id：
• 3981

• Human Swiss Prot No：
• P49917

• Mouse Swiss Prot No：
• Q8BTF7

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human DNA Ligase 4 around the phosphorylation site of Thr650. AA range:616-665

• 特异性：
• Phospho-DNA Ligase IV (T650) Polyclonal Antibody detects endogenous levels of DNA Ligase IV protein only when phosphorylated at T650.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• LIG4;DNA ligase 4;DNA ligase IV;Polydeoxyribonucleotide synthase [ATP] 4

• 分子量：
• 104kD

• 背景：
• The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008],

• 功能：
• catalytic activity:ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).,cofactor:Magnesium.,disease:Defects in LIG4 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive with sensitivity to ionizing radiation (RSSCID) [MIM:602450]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Individuals affected by RS-SCID show defects in the DNA repair machinery necessary for coding joint

• 细胞定位：
• Nucleus .

• 组织表达：
• Testis, thymus, prostate and heart.

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