• 靶点：
• MKP-1

• 简介：
• >>MAPK signaling pathway;>>Serotonergic synapse;>>Parkinson disease;>>Fluid shear stress and atherosclerosis

• 基因名称：
• DUSP1

• 蛋白名称：
• Dual specificity protein phosphatase 1

• Human Gene Id：
• 1843

• Human Swiss Prot No：
• P28562

• Mouse Swiss Prot No：
• P28563

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human MKP1 around the phosphorylation site of Ser359. AA range:318-367

• 特异性：
• Phospho-MKP-1 (S359) Polyclonal Antibody detects endogenous levels of MKP-1 protein only when phosphorylated at S359.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• DUSP1;CL100;MKP1;PTPN10;VH1;Dual specificity protein phosphatase 1;Dual specificity protein phosphatase hVH1;Mitogen-activated protein kinase phosphatase 1;MAP kinase phosphatase 1;MKP-1;Protein-tyrosine phosphatase CL100

• 分子量：
• 39kD

• 背景：
• The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferati

• 功能：
• catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,function:Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'.,induction:By oxidative stress and heat shock.,similarity:Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.,similarity:Contains 1 rhodanese domain.,similarity:Contains 1 tyrosine-protein phosphatase domain.,tissue specificity:Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.,

• 细胞定位：
• Nucleus .

• 组织表达：
• Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.

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