• 靶点：
• IRP-1

• 简介：
• >>Citrate cycle (TCA cycle);>>Glyoxylate and dicarboxylate metabolism;>>Metabolic pathways;>>Carbon metabolism;>>2-Oxocarboxylic acid metabolism;>>Biosynthesis of amino acids

• 基因名称：
• ACO1

• 蛋白名称：
• Cytoplasmic aconitate hydratase

• Human Gene Id：
• 48

• Human Swiss Prot No：
• P21399

• Mouse Gene Id：
• 11428

• Mouse Swiss Prot No：
• P28271

• Rat Gene Id：
• 50655

• Rat Swiss Prot No：
• Q63270

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human IREB1 around the phosphorylation site of Ser138. AA range:106-155

• 特异性：
• Phospho-IRP-1 (S138) Polyclonal Antibody detects endogenous levels of IRP-1 protein only when phosphorylated at S138.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• ACO1;IREB1;Cytoplasmic aconitate hydratase;Aconitase;Citrate hydro-lyase;Ferritin repressor protein;Iron regulatory protein 1;IRP1;Iron-responsive element-binding protein 1;IRE-BP 1

• 分子量：
• 98kD

• 背景：
• The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct

• 功能：
• catalytic activity:Citrate = isocitrate.,cofactor:Binds 1 4Fe-4S cluster per subunit.,function:Binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. This protein also expresses aconitase activity.,online information:Aconitase entry,similarity:Belongs to the aconitase/IPM isomerase family.,

• 细胞定位：
• Cytoplasm, cytosol .

• 组织表达：
• Brain,Brain astrocytoma,Uterus,

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