CaMKIα (phospho Thr177) Polyclonal Antibody
- 货号:YP0913
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 靶点:
- CaMKIα
- 简介:
- >>Calcium signaling pathway;>>Oxytocin signaling pathway;>>Aldosterone synthesis and secretion;>>Glioma
- 基因名称:
- CAMK1
- 蛋白名称:
- Calcium/calmodulin-dependent protein kinase type 1
- Human Gene Id:
- 8536
- Human Swiss Prot No:
- Q14012
- Mouse Gene Id:
- 52163
- Mouse Swiss Prot No:
- Q91YS8
- Rat Gene Id:
- 171503
- Rat Swiss Prot No:
- Q63450
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human CaMK1-alpha around the phosphorylation site of Thr177. AA range:143-192
- 特异性:
- Phospho-CaMKIα (T177) Polyclonal Antibody detects endogenous levels of CaMKIα protein only when phosphorylated at T177.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- CAMK1;Calcium/calmodulin-dependent protein kinase type 1;CaM kinase I;CaM-KI;CaM kinase I alpha;CaMKI-alpha
- 实测条带:
- 41kD
- 背景:
- Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.,enzyme regulation:Activated by Ca(2+)/calmodulin. Binding of calmodulin results in a conformational change that generates functional binding sites for both, substrate and ATP, and thus releaves intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2.,function:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes like transcriptional regulation, hormone production, translational regulation, regulation of actin filament organization and neurite outgrowth. Involved in calcium-dependent activation of the ERK pathway (By si
- 细胞定位:
- Cytoplasm . Nucleus . Predominantly cytoplasmic. .
- 组织表达:
- Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
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- July 13-2018
- Antibody-FAQs
- 产品图片
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CaMK1-alpha (Phospho-Thr177) Antibody
- Immunofluorescence analysis of HeLa cells, using CaMK1-alpha (Phospho-Thr177) Antibody. The picture on the right is blocked with the phospho peptide.
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CaMK1-alpha (Phospho-Thr177) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from K562 cells treated with insulin 0.01U/ml 15' and Jurkat cells treated with insulin 0.01U/ml 15', using CaMK1-alpha (Phospho-Thr177) Antibody. The lane on the right is blocked with the phospho peptide.