• 靶点：
• RARα

• 简介：
• >>Th17 cell differentiation;>>Estrogen signaling pathway;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Acute myeloid leukemia

• 基因名称：
• RARA

• 蛋白名称：
• Retinoic acid receptor alpha

• Human Gene Id：
• 5914

• Human Swiss Prot No：
• P10276

• Mouse Gene Id：
• 19401

• Mouse Swiss Prot No：
• P11416

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human Retinoic Acid Receptor alpha around the phosphorylation site of Ser77. AA range:46-95

• 特异性：
• Phospho-RARα (S77) Polyclonal Antibody detects endogenous levels of RARα protein only when phosphorylated at S77.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• RARA;NR1B1;Retinoic acid receptor alpha;RAR-alpha;Nuclear receptor subfamily 1 group B member 1

• 实测条带：
• 45kD

• 背景：
• This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.[provided by RefSeq, Sep 2010],

• 功能：
• disease:Chromosomal aberrations involving RARA may be a cause of acute promyelocytic leukemia (APL) [MIM:612376]. Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM.,domain:Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.,function:This is a receptor for retinoic acid. This metabolite has profound effects on vertebrate development. Retinoic acid is a morphogen and is a powerful teratogen. This receptor controls cell function by directly regulating gene expression.,online information:Retinoic acid receptor entry,PTM:Phosphorylated. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity.,similarity:Belongs to the nuclear hormone receptor family.,similarity:Belongs to the nuclear

• 细胞定位：
• Nucleus . Cytoplasm . Nuclear localization depends on ligand binding, phosphorylation and sumoylation (PubMed:19850744). Translocation to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation (By similarity). Increased nuclear localization upon pulsatile shear stress (PubMed:28167758). .

• 组织表达：
• Expressed in monocytes.

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