MSK1 (phospho Ser212) Polyclonal Antibody
- 货号:YP0791
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 靶点:
- MSK1
- 简介:
- >>MAPK signaling pathway;>>Adrenergic signaling in cardiomyocytes;>>TNF signaling pathway;>>Circadian entrainment;>>Neurotrophin signaling pathway;>>Shigellosis;>>Pathways in cancer;>>MicroRNAs in cancer;>>Bladder cancer
- 基因名称:
- RPS6KA5
- 蛋白名称:
- Ribosomal protein S6 kinase alpha-5
- Human Gene Id:
- 9252
- Human Swiss Prot No:
- O75582
- Mouse Gene Id:
- 73086
- Mouse Swiss Prot No:
- Q8C050
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human MSK1 around the phosphorylation site of Ser212. AA range:181-230
- 特异性:
- Phospho-MSK1 (S212) Polyclonal Antibody detects endogenous levels of MSK1 protein only when phosphorylated at S212.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- RPS6KA5;MSK1;Ribosomal protein S6 kinase alpha-5;S6K-alpha-5;90 kDa ribosomal protein S6 kinase 5;Nuclear mitogen- and stress-activated protein kinase 1;RSK-like protein kinase;RSKL
- 实测条带:
- 90kD
- 背景:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Appears to be activated by multiple phosphorylations on threonine and serine residues. ERK1/2 and MAPK14/p38-alpha may play a role in this process.,function:Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).,miscellaneous:Enzyme activity requires the presence of both kinase domains.,PTM:Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 2 protein kinase domains.,subcellular location:Predominantly nuclear. Partially cytoplasmic.,subunit:Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA5 associates with and phosphorylates the NF-kappa-B p65 subunit RELA.,tissue specificity:Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.,
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Appears to be activated by multiple phosphorylations on threonine and serine residues. ERK1/2 and MAPK14/p38-alpha may play a role in this process.,function:Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28'
- 细胞定位:
- Nucleus. Cytoplasm. Predominantly nuclear. Exported into cytoplasm in response to glucocorticoid.
- 组织表达:
- Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
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- Antibody-FAQs
- 产品图片
- Western Blot analysis of 3T3 cells using Phospho-MSK1 (S212) Polyclonal Antibody diluted at 1:1000
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MSK1 (Phospho-Ser212) Antibody
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MSK1 (Phospho-Ser212) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 5', using MSK1 (Phospho-Ser212) Antibody. The lane on the right is blocked with the phospho peptide.