MEK Kinase-1 (phospho Thr1402) Polyclonal Antibody
- 货号:YP0786
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 靶点:
- MEK Kinase-1
- 简介:
- >>MAPK signaling pathway;>>Ubiquitin mediated proteolysis;>>Tight junction;>>RIG-I-like receptor signaling pathway;>>Neurotrophin signaling pathway;>>GnRH signaling pathway;>>Growth hormone synthesis, secretion and action;>>Hepatitis B;>>Human T-cell leukemia virus 1 infection
- 基因名称:
- MAP3K1
- 蛋白名称:
- Mitogen-activated protein kinase kinase kinase 1
- Human Gene Id:
- 4214
- Human Swiss Prot No:
- Q13233
- Mouse Swiss Prot No:
- P53349
- Rat Gene Id:
- 116667
- Rat Swiss Prot No:
- Q62925
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human MAP3K1 around the phosphorylation site of Thr1402. AA range:1368-1417
- 特异性:
- Phospho-MEK Kinase-1 (T1402) Polyclonal Antibody detects endogenous levels of MEK Kinase-1 protein only when phosphorylated at T1402.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- MAP3K1;MAPKKK1;MEKK;MEKK1;Mitogen-activated protein kinase kinase kinase 1;MAPK/ERK kinase kinase 1;MEK kinase 1;MEKK 1
- 实测条带:
- 160kD
- 背景:
- The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq, Mar 2012],
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by autophosphorylation on Thr-1400 and Thr-1412 following oligomerization.,function:Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 1 SWIM-type zinc finger.,subunit:Binds both upstream activators and downstream substrates in multimolecular complexes through its N-terminus. Oligomerizes after binding MAP4K2 or TRAF2. Interacts with AXIN1.,
- 细胞定位:
- cytoplasm,cytosol,
- 组织表达:
- Leukocyte,
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- Antibody-FAQs
- 产品图片
- Western Blot analysis of NIH-3T3 cells using Phospho-MEK Kinase-1 (T1402) Polyclonal Antibody diluted at 1:2000
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAP3K1 (Phospho-Thr1402) Antibody
- Immunohistochemistry analysis of paraffin-embedded human brain, using MAP3K1 (Phospho-Thr1402) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from Jurkat cells, using MAP3K1 (Phospho-Thr1402) Antibody. The lane on the right is blocked with the phospho peptide.