• 靶点：
• PKC θ

• 简介：
• >>NF-kappa B signaling pathway;>>Autophagy - animal;>>Vascular smooth muscle contraction;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>Inflammatory mediator regulation of TRP channels;>>Adipocytokine signaling pathway;>>Insulin resistance;>>Shigellosis;>>PD-L1 expression and PD-1 checkpoint pathway in cancer

• 基因名称：
• PRKCQ

• 蛋白名称：
• Protein kinase C theta type

• Human Gene Id：
• 5588

• Human Swiss Prot No：
• Q04759

• Mouse Gene Id：
• 18761

• Mouse Swiss Prot No：
• Q02111

• Rat Swiss Prot No：
• Q9WTQ0

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human PKC thet around the phosphorylation site of Thr538. AA range:504-553

• 特异性：
• Phospho-PKC θ (T538) Polyclonal Antibody detects endogenous levels of PKC θ protein only when phosphorylated at T538.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• PRKCQ;PRKCT;Protein kinase C theta type;nPKC-theta

• 实测条带：
• 81kD

• 背景：
• Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008],

• 功能：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.,enzyme regulation:Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation.,function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.,function:This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activ

• 细胞定位：
• Cytoplasm. Cell membrane; Peripheral membrane protein. In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.

• 组织表达：
• Expressed in skeletal muscle, T-cells, megakaryoblastic cells and platelets.

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