• 靶点：
• ATP-citrate synthase

• 简介：
• >>Citrate cycle (TCA cycle);>>Metabolic pathways

• 基因名称：
• ACLY

• 蛋白名称：
• ATP-citrate synthase

• Human Gene Id：
• 47

• Human Swiss Prot No：
• P53396

• Mouse Gene Id：
• 104112

• Mouse Swiss Prot No：
• Q91V92

• Rat Gene Id：
• 24159

• Rat Swiss Prot No：
• P16638

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human ATP-Citrate Lyase around the phosphorylation site of Ser454. AA range:420-469

• 特异性：
• Phospho-ATP-citrate synthase (S455) Polyclonal Antibody detects endogenous levels of ATP-citrate synthase protein only when phosphorylated at S455.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• ACLY;ATP-citrate synthase;ATP-citrate;pro-S-)-lyase;ACL;Citrate cleavage enzyme

• 实测条带：
• 125kD

• 背景：
• ATP citrate lyase(ACLY) Homo sapiens ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014],

• 功能：
• catalytic activity:ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA.,function:ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.,similarity:In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.,similarity:In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.,subunit:Homotetramer.,

• 细胞定位：
• Cytoplasm, cytosol .

• 组织表达：
• Brain,Epithelium,Hippocampus,Liver,Lymph,Platelet,

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