• 靶点：
• ALK

• 简介：
• >>Pathways in cancer;>>Non-small cell lung cancer;>>PD-L1 expression and PD-1 checkpoint pathway in cancer

• 基因名称：
• ALK

• 蛋白名称：
• ALK tyrosine kinase receptor

• Human Gene Id：
• 238

• Human Swiss Prot No：
• Q9UM73

• Mouse Gene Id：
• 11682

• Mouse Swiss Prot No：
• P97793

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human ALK around the phosphorylation site of Tyr1507. AA range:1473-1522

• 特异性：
• Phospho-ALK (Y1507) Polyclonal Antibody detects endogenous levels of ALK protein only when phosphorylated at Y1507.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• ALK;ALK tyrosine kinase receptor;Anaplastic lymphoma kinase;CD antigen CD246

• 实测条带：
• 150-240kD

• 背景：
• This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome

• 功能：
• catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.,disease:A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A.,disease:A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas.,function:Orphan receptor with a tyrosine-protein kinase activity. Appears to play an important role in the normal development and function

• 细胞定位：
• Cell membrane ; Single-pass type I membrane protein . Membrane attachment is essential for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway. .

• 组织表达：
• Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.

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