• 靶点：
• Nek9

• 基因名称：
• NEK9

• 蛋白名称：
• Serine/threonine-protein kinase Nek9

• Human Gene Id：
• 91754

• Human Swiss Prot No：
• Q8TD19

• Mouse Gene Id：
• 217718

• Mouse Swiss Prot No：
• Q8K1R7

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human NEK9 around the phosphorylation site of Thr210. AA range:176-225

• 特异性：
• Phospho-Nek9 (T210) Polyclonal Antibody detects endogenous levels of Nek9 protein only when phosphorylated at T210.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• NEK9;KIAA1995;NEK8;NERCC;Serine/threonine-protein kinase Nek9;Nercc1 kinase;Never in mitosis A-related kinase 9;NimA-related protein kinase 9;NimA-related kinase 8;Nek8

• 实测条带：
• 110kD

• 背景：
• This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein is activated in mitosis and, in turn, activates other family members during mitosis. This protein also mediates cellular processes that are essential for interphase progression. [provided by RefSeq, Jul 2016],

• 功能：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,developmental stage:Expression varied mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.,domain:Dimerizes through its coiled-coil domain.,enzyme regulation:Activated during mitosis by intramolecular autophosphorylation. Activity and autophosphorylation is activated by manganese >> magnesium ions. Sensitive to increasing concentration of detergents. It is not cell-cycle regulated but activity is higher in G0-arrested cells.,function:Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S pha

• 细胞定位：
• Cytoplasm . Nucleus .

• 组织表达：
• Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.

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