• 靶点：
• EphB1/2

• 简介：
• >>Axon guidance

• 基因名称：
• EPHB1/EPHB2

• 蛋白名称：
• Ephrin type-B receptor 1/2

• Human Gene Id：
• 2047/1969

• Human Swiss Prot No：
• P54762/P29323

• Mouse Gene Id：
• 270190

• Rat Gene Id：
• 24338

• Rat Swiss Prot No：
• P09759

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human EPHB1/2 around the phosphorylation site of Tyr594/604. AA range:561-610

• 特异性：
• Phospho-EphB1/2 (Y594/604) Polyclonal Antibody detects endogenous levels of EphB1/2 protein only when phosphorylated at Y594/604.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• EPHB1;ELK;EPHT2;HEK6;NET;Ephrin type-B receptor 1;ELK;EPH tyrosine kinase 2;EPH-like kinase 6;EK6;hEK6;Neuronally-expressed EPH-related tyrosine kinase;NET;Tyrosine-protein kinase receptor EPH-2;EPHB2;DRT;EPHT3;EPTH3;ERK;

• 实测条带：
• 110kD

• 背景：
• Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008],

• 功能：
• catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Receptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. May be involved in cell-cell interactions in the nervous system.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 fibronectin type-III domains.,subunit:The ligand-activated form interacts with GRB2, GRB10 and NCK through their respective SH2 domains. The GRB10 SH2 domain binds EPHB1 through Tyr-928, while GRB2 binds residues within the catalytic domain. Interacts with EPHB6. The NCK SH2 domain binds EPHB1 through Tyr-594. Interacts with PRKCABP.,tissue specificity:Preferentially expressed in brain.,

• 细胞定位：
• Cell membrane ; Single-pass type I membrane protein . Early endosome membrane . Cell projection, dendrite .

• 组织表达：
• Preferentially expressed in brain.

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