• 靶点：
• EphA2/3/4

• 简介：
• >>MAPK signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>PI3K-Akt signaling pathway;>>Axon guidance

• 基因名称：
• EPHA2/3/4

• 蛋白名称：
• Ephrin type-A receptor 2/3/4

• Human Gene Id：
• 1969/2042/2043

• Human Swiss Prot No：
• P29317/P29320/P54764

• Mouse Gene Id：
• 13836/13838

• Rat Gene Id：
• 29210

• Rat Swiss Prot No：
• O08680

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human EPHA2/3 around the phosphorylation site of Tyr588/596. AA range:556-605

• 特异性：
• Phospho-EphA2/3/4 (Y588/596) Polyclonal Antibody detects endogenous levels of EphA2/3/4 protein only when phosphorylated at Y588/596.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• EPHA2;ECK;Ephrin type-A receptor 2;Epithelial cell kinase;Tyrosine-protein kinase receptor ECK;EPHA3;ETK;ETK1;HEK;TYRO4;Ephrin type-A receptor 3;EPH-like kinase 4;EK4;hEK4;HEK;Human embryo kinase;Tyrosine-protein kinase TYRO

• 实测条带：
• 130kD

• 背景：
• This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010],

• 功能：
• catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 fibronectin type-III domains.,subunit:Interacts with SLA (By similarity). Interacts with INPPL1/SHIP2.,tissue specificity:Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary.,

• 细胞定位：
• Cell membrane ; Single-pass type I membrane protein . Cell projection, ruffle membrane ; Single-pass type I membrane protein . Cell projection, lamellipodium membrane ; Single-pass type I membrane protein . Cell junction, focal adhesion . Present at regions of cell-cell contacts but also at the leading edge of migrating cells (PubMed:19573808, PubMed:20861311). Relocates from the plasma membrane to the cytoplasmic and perinuclear regions in cancer cells (PubMed:18794797). .

• 组织表达：
• Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g. skin, intestine, lung, and ovary.

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